Multiplex PCR to Detect Phage Excision
Corresponding Organization :
Other organizations : University of Montana, Stanford University, Children's Hospital of Los Angeles, University of Southern California, Amrita Vishwa Vidyapeetham
Variable analysis
- Presence or absence of Pf prophage integration
- Amplicon size (smaller band for integration, larger band for excision)
- Phusion Plus PCR Mastermix (Thermo Scientific # F631L)
- Primer concentration (0.5 µM)
- Positive control: Amplicons produced when Pf prophage is integrated (smaller band)
- Negative control: Amplicons produced when Pf prophage is excised (larger band)
Annotations
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