Female NOD/ShiLtJ (NOD), NOD.CB17-Prkdcscid/J (NOD-scid), B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6g7), and DBA/2J (DBA) mice were from The Jackson Laboratory. B6.G9C8 mice, transgenic for T-cell receptor (TCR) derived from InsB15–23-specific CD8+ T-cell clone G9C8, and H-2Kd MHC allele (19 (link)) were provided by Dr. A. Chervonsky (University of Chicago, Chicago, IL).