DNA was isolated from peripheral blood collected from the donor and recipient prior to transplantation. Lymphocytes were isolated by centrifugation after red blood cell lysis. Isolated DNA was quantified at 260 nm absorbance. Single nucleotide polymorphisms were genotyped using a customized Affymetrix GeneChip and additional SNPs were measured using SNPlex (Applied Biosystems Inc, Foster City, California, USA) and Sequenom (Sequenom, Inc, San Diego, CA, USA) systems as previously described 19 (link). Candidate polymorphisms (n=88) measured in both the donor and recipient potentially relevant to mycophenolate metabolism, transport and mechanism of action were selected for this analysis (Table S1 ).
Protocol detail
SNP Genotyping for Transplant Pharmacogenomics
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Blood
Centrifugation
Donor
Genechip
Lymphocytes
Mechanism of action
Metabolism
Polymorphisms
Red blood cell
Snps
Transplantation recipient
Corresponding Organization : GlaxoSmithKline (United States)
Other organizations : University of Minnesota, Hennepin County Medical Center
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Variable analysis
independent variables
- DNA isolated from peripheral blood of the donor
- DNA isolated from peripheral blood of the recipient
- Single nucleotide polymorphisms genotyped using a customized Affymetrix GeneChip
- Additional single nucleotide polymorphisms measured using SNPlex (Applied Biosystems Inc) and Sequenom (Sequenom, Inc) systems
- Lymphocytes isolated by centrifugation after red blood cell lysis
- Isolated DNA quantified at 260 nm absorbance
- No positive or negative controls explicitly mentioned
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