In Vitro BBB Modeling with hBMECs

All reagents were purchased from Millipore-Sigma (Burlington, MA, USA), unless otherwise stated. Human brain microvascular endothelial cells (hBMECs) were obtained from Neuromics (Minneapolis, MN; catalog number: #HEC02). These cells have been proved to be the most suitable human cell line for an in vitro blood–brain barrier (BBB) model [29 (link)].
Cells were cultured in a standard humidified atmosphere (37°C) containing 5% CO₂. In some experiments, cells were transfected with pcDNA3.1-Neuropilin-1 plasmids (GenScript, Piscataway, NJ).

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Mone P., Gambardella J., Wang X., Jankauskas S.S., Matarese A, & Santulli G. (2021). miR-24 targets SARS-CoV-2 co-factor Neuropilin-1 in human brain microvascular endothelial cells: Insights for COVID-19 neurological manifestations. Research Square.

Publication Preprint 2021

hBMECs pcDNA3.1-Neuropilin-1 plasmids

Atmosphere Brain Cell line Cells Endothelial cells Human Neuropilin 1 Plasmids

Corresponding Organization :

Other organizations : Albert Einstein College of Medicine

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Variable analysis

independent variables

Transfection with pcDNA3.1-Neuropilin-1 plasmids

dependent variables

Unspecified

control variables

Culturing cells in a standard humidified atmosphere (37°C) containing 5% CO2

controls

Positive control: Not specified
Negative control: Not specified

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