A subgroup of patients (n = 192) underwent blood collection for serum NfL measurement; 63 healthy controls (HC) (age 67.0, IQR 61.0–74.0 years) were recruited among spouses or caregivers as reference.
Serum was collected by venipuncture, processed and stored in aliquots at – 80 °C according to standardized procedures. Serum NfL was measured by single-molecule array (Simoa) technology on an HD-X Analyzer using the commercial NF-light® assay according to the manufacturer’s instructions (Quanterix, Billerica, MA). Detailed analytical procedures and assay validation have been previously described [15 (link)]. The lower limits of quantitation for serum NfL were 0.174 pg/mL. The measurements were performed out in one round of experiments using the same batch of reagents, and the operators were blinded to all clinical information. Quality control samples had intra-assay and inter-assay coefficients of variation of less than 8 and 20%, respectively.
Serum was collected by venipuncture, processed and stored in aliquots at – 80 °C according to standardized procedures. Serum NfL was measured by single-molecule array (Simoa) technology on an HD-X Analyzer using the commercial NF-light® assay according to the manufacturer’s instructions (Quanterix, Billerica, MA). Detailed analytical procedures and assay validation have been previously described [15 (link)]. The lower limits of quantitation for serum NfL were 0.174 pg/mL. The measurements were performed out in one round of experiments using the same batch of reagents, and the operators were blinded to all clinical information. Quality control samples had intra-assay and inter-assay coefficients of variation of less than 8 and 20%, respectively.
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