MMP2/9 activities were assayed using gelatin zymogram assay, as described previously [80 (link)]. HaCaT cells were treated with diluted LP to 1/2, 1/4, incubated for an additional 24 h. The supernatant (100 μL) from each sample was mixed with 4-aminophenylmercuric acetate (100 μM, Sigma-Aldrich, St Louis, MO, USA), and the samples were activated for 1 h at 37 °C. The sample was placed in sample buffer for 10 min and electrophoresed on a polyacrylamide gel at 125 V for 120 min at 4 °C using a Novex XCell II system (Life Technologies, Carlsbad, CA, USA). Digital images were taken using an image analyzer.
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