Propranolol [(±)-Propranolol hydrochloride, Sigma-Aldrich], was dissolved in 0.9% sodium chloride solution and administered 10 mg/kg intra peritoneally (i.p.) at 0, 4 and 8 h after tMCAO as previously described.24 (link) The control animals were treated with sodium chloride 0.9% (saline) i.p. at the corresponding time points.
FTY720 (Cayman Chemicals) was dissolved in 0.9% sodium chloride solution (saline) at a final concentration of 10 µg/100 µl for i.p. injection. FTY720 (1 mg/kg) was administered i.p. after the initiation of anesthesia as previously described.10 (link) The control animals were treated with corresponding volumes of saline i.p. All treatments were administered by RV, who was not involved in the tMCAO operation and further sample processing and analyses.
FTY720 (Cayman Chemicals) was dissolved in 0.9% sodium chloride solution (saline) at a final concentration of 10 µg/100 µl for i.p. injection. FTY720 (1 mg/kg) was administered i.p. after the initiation of anesthesia as previously described.10 (link) The control animals were treated with corresponding volumes of saline i.p. All treatments were administered by RV, who was not involved in the tMCAO operation and further sample processing and analyses.
