Histochemical Analysis of Skin Samples

For histochemistry and immune‐histochemistry, paraffin‐embedded skin samples were cut into 4 μm sections. Haematoxylin and eosin (HE) staining using Mayer's Hemalaun (1.09249.2500, Merck) and Eosin Y (1.15935.0100, Merck) was done in a linear slide stainer (Leica ST4040). For Masson–Goldner trichrome staining, kits were used according to the manufacturer's recommendations (12043, 14604, Morhisto). Automatic scanning of full slides in 40x magnification was done using the VS‐120‐L Olympus slide scanner 100‐W system and processed using the Olympus VS‐ASW‐L100 program. Evaluation of the epidermal thickness and murine vessel quantification was done according to published work (Ebner‐Peking et al., 2021 (link)), both measured in the Olympus VS‐ASM‐L100 program. Per group, four biological and at least three technical replicates were included.

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Wolf M., Poupardin R.W., Ebner‐Peking P., Andrade A.C., Blöchl C., Obermayer A., Gomes F.G., Vari B., Maeding N., Eminger E., Binder H., Raninger A.M., Hochmann S., Brachtl G., Spittler A., Heuser T., Ofir R., Huber C.G., Aberman Z., Schallmoser K., Volk H, & Strunk D. (2022). A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation. Journal of Extracellular Vesicles, 11(4), e12207.

Publication 2022

Mayer's Hemalaun Eosin Y ST4040 VS‐120‐L Olympus slide scanner 100‐W system VS‐ASW‐L100 program

Biological Eosin y Epidermal Haematoxylin Histochemistry Murine Paraffin Skin Vessel

Corresponding Organization : Paracelsus Medical University

Other organizations : University of Salzburg, Medical University of Vienna, Vienna Biocenter, Pluristem Therapeutics (Israel), Berlin Institute of Health at Charité - Universitätsmedizin Berlin

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Variable analysis

independent variables

Type of staining (Haematoxylin and eosin (HE) staining, Masson–Goldner trichrome staining)

dependent variables

Epidermal thickness
Murine vessel quantification

control variables

Paraffin-embedded skin samples
4 μm sections
Mayer's Hemalaun (1.09249.2500, Merck) and Eosin Y (1.15935.0100, Merck) for HE staining
Masson–Goldner trichrome staining kits (12043, 14604, Morhisto)
Automatic scanning at 40x magnification using Olympus VS‐120‐L slide scanner
Evaluation of epidermal thickness and murine vessel quantification using Olympus VS‐ASM‐L100 program
Four biological replicates and at least three technical replicates per group

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