Cytotoxicity Evaluation of Chalcone Derivatives

Cell viability was determined by 2,3-bis[2-methoxy-4-nitro- 5-sulfophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT) assay (Roche Diagnostics GmbH, Mannheim, Germany). Cells seeded at the concentration of 1,000 per well in 100 µL medium in 96-well plate were treated with chalcone-based derivatives at specified concentrations. After the indicated periods, the cells were incubated according to the manufacturer's protocol with the XTT labeling mixture for 4 hours. Formazan dye was quantified using a spectrophotometric plate reader to measure the absorbance at 450 nm (ELISA reader 190; Molecular Devices, Sunnyvale, CA). All experiments were done in triplicate.