Flow cytometry for immune cell profiling

For immune cell profiling, ∼1,000,000 cells per mouse were stained with fluorochrome-conjugated antibodies against CD45, CD3, CD4, CD8, FOXP3, RORγt, and CD62L (eBioscience), and their populations were analyzed by a CytoFLEX (Beckman-Coulter) using software packages from CellQuest and FlowJo version 12 as described previously (35 (link)).

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Henke M.T., Brown E.M., Cassilly C.D., Vlamakis H., Xavier R.J, & Clardy J. (2021). Capsular polysaccharide correlates with immune response to the human gut microbe Ruminococcus gnavus. Proceedings of the National Academy of Sciences of the United States of America, 118(20), e2007595118.

Publication 2021

fluorochrome-conjugated antibodies against CD45 RORγt CytoFLEX

Antibodies Cd62l Cells Fluorochrome Mouse Populations Rorγt

Corresponding Organization :

Other organizations : Harvard University, Massachusetts General Hospital, Broad Institute

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Variable analysis

independent variables

Staining with fluorochrome-conjugated antibodies against CD45, CD3, CD4, CD8, FOXP3, RORγt, and CD62L

dependent variables

Immune cell populations analyzed by a CytoFLEX (Beckman-Coulter) using software packages from CellQuest and FlowJo version 12

control variables

Not explicitly mentioned

controls

Not explicitly mentioned

Annotations

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