Human pancreatic cancer tissue array was purchased from US Biomax as described above. PDX-1-Cre:LSL-KRASG12D mouse pancreas sections were provided by A. M. Lowy. Samples from patients BK-17 and BK-19 were collected and processed as described previously [30 (link)], in accordance with UCSD (La Jolla, CA) Institutional Review Board (IRB) #071136X. Determination of eIF5A1, eIF5A2 and hypusinated eIF5A1 protein expression was performed as described previously using specific antibodies (ab32443 (Abcam) for eIF5A1, H00056648-M01 (Abnova) for eIF5A2 and NIH353 for hypusinated eIF5A1) [30 (link), 35 (link), 36 (link)]. Images were collected with a Leica DM2500 microscope using a 40x objective lens.
Protocol detail
Immunohistochemical Analysis of eIF5A in Pancreatic Cancer
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Antibodies
Human
Institutional review board
Lens
Microscope
Mouse
Pancreas
Pancreatic cancer
Patients
Pdx 1
Protein
Tissue
Corresponding Organization : University of California, San Diego
Other organizations : California State University, Northridge
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Variable analysis
independent variables
- Tissue type (human pancreatic cancer tissue array and PDX-1-Cre:LSL-KRAS^G12D^ mouse pancreas sections)
- EIF5A1 protein expression
- EIF5A2 protein expression
- Hypusinated eIF5A1 protein expression
- Antibodies used for protein expression determination (ab32443 (Abcam) for eIF5A1, H00056648-M01 (Abnova) for eIF5A2, and NIH353 for hypusinated eIF5A1)
- Microscope and objective lens (Leica DM2500 microscope with a 40x objective lens)
- Samples from patients BK-17 and BK-19 were collected and processed as described previously [30 (link)]
- No negative controls were explicitly mentioned.
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