SEC-MALLS analysis of DnaADI and SirA–DnaADI was carried out at a range of protein concentrations: DnaADI was analysed at 1.0 mg ml−1, 2.5 mg ml−1 and 5.0 mg ml−1 and SirA–DnaADI was analysed at 0.5 mg ml−1, 1.0 mg ml−1 and 2.5 mg ml−1. For each run, 100 μl of sample was loaded onto a Superdex 75 HR 10/30 size-exclusion column equilibrated with 50 mM Tris pH 8.5, 200 mM KCl at a flow rate of 0.5 ml min−1. The eluate was analysed successively by a SPD20A UV/Vis detector, a Wyatt Dawn HELEOS-II 18-angle light scattering detector and a Wyatt Optilab rEX refractive index monitor as described previously (Colledge et al., 2011 (link)). Data were analysed with Astra software (Wyatt).
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