Human brain microvascular ECs (ScienCell and PromoCell, isolated from adult or fetal human brains) and GBM-derived ECs were maintained as previously described13 (link),14 (link). In brief, cells were cultured in Endothelial Cell Medium (ScienCell, supplemented with vascular endothelial growth factor A) at 37°C with 5% CO2. All cells were used between passages two and five. Cells were treated with KPT9274 or PF3758309 (Selleckchem).
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Cultivation and Treatment of Human Brain Microvascular Endothelial Cells
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Other organizations : University of Pennsylvania
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Variable analysis
independent variables
- KPT9274
- PF3758309
- Not explicitly mentioned
- Passage number (two to five)
- Culture conditions (37°C with 5% CO2)
- Culture medium (Endothelial Cell Medium supplemented with vascular endothelial growth factor A)
- Positive control: Not specified
- Negative control: Not specified
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