Transient Expression of GFP-TaATG8h and pAN583:TaCDPK27-mCherry in Wheat Protoplasts

GFP-TaATG8h-expressing vectors were constructed according to the methods of Pei et al. (2014) [49 (link)]. A pAN583:TaCDPK27-mCherry-expressing vector was constructed as described by Ni et al. (2022) [50 (link)]. The primers used are listed in Table 1. The PEG-mediated transfection method was employed to transform the TaCDPK27-mCherry vector and GFP-TaATG8h-expressing vectors into wheat protoplasts [50 (link)]. Then, the mCherry and GFP signals were observed using a confocal laser scanning microscope (Nikon Ti2 Eclipse A1).

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Yue J.Y., Jiao J.L., Wang W.W., Jie X.R, & Wang H.Z. (2023). Silencing of the calcium-dependent protein kinase TaCDPK27 improves wheat resistance to powdery mildew. BMC Plant Biology, 23, 134.

Publication 2023

Ti2 Eclipse A1

Confocal laser scanning microscope Primers Protoplasts Transfection Vectors Wheat

Corresponding Organization : Tianjin Normal University

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Variable analysis

independent variables

GFP-TaATG8h-expressing vectors
TaCDPK27-mCherry-expressing vector

dependent variables

MCherry and GFP signals

control variables

Wheat protoplasts

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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