All DNAs were ordered from Tsingke Biotechnology (Beijing, China). Fluorescence was measured on a FluoroMax + spectrofluorometer (Horiba Scientific) using FluorEssence software (v.3.9). Fluorescence imaging was acquired on an Olympus SpinSR10 microscope (Olympus). Data were plotted using Origin 2021 software. All RNA-binding fluorophores, including HBC, HBC620, BI, DFHO and tetramethyl rhodamine-dinitroaniline (TMR-DN) used in this study were synthesized as described previously (25 (link),34 (link),35 (link)).
Protocol detail
RNA-Binding Fluorophore Synthesis Protocols
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Chinese Academy of Sciences, Affiliated Hospital of Southwest Medical University, University of Chinese Academy of Sciences, University of South China, Hebei University, University of Massachusetts Amherst, Tianjin Institute of Industrial Biotechnology, Institute of Zoology
Top 5 similar protocols
Variable analysis
independent variables
- RNA-binding fluorophores, including HBC, HBC620, BI, DFHO and tetramethyl rhodamine-dinitroaniline (TMR-DN)
- Fluorescence measured on a FluoroMax + spectrofluorometer
- Fluorescence imaging acquired on an Olympus SpinSR10 microscope
- All DNAs were ordered from Tsingke Biotechnology (Beijing, China)
- Fluorescence was measured using FluorEssence software (v.3.9)
- Data were plotted using Origin 2021 software
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!