Endometrial Expression Profiling in Recurrent Implantation Failure

We input “recurrent implantation failure” and “expression profile” as two keywords to the GEO database, then two datasets GSE103465 and GSE111974 were selected for analysis, and GSE26787 was chosen for validation. Both GSE103465 and GSE111974 contain expression profiles of endometrial tissue obtained from RIF and control women during WOI. GSE103465, in GPL16043 platform, contains whole-genome expression profiles of endometrial tissue from three women divided to the control group and RIF group (Guo et al., 2018 (link)). In GSE103465, RIF is defined as no pregnancy after ≥3 embryo transfers including a total of ≥4 good-quality embryos, and inclusion criteria of control group is infertile women with tubal factors who achieved a clinical pregnancy after the first embryo transfer (Guo et al., 2018 (link)). GSE111974, in GPL17077 platform, consists of 24 individuals with RIF and 24 fertile control patients, in which RIF is determined as failure of pregnancy in three consecutive IVF cycles with at least one transfer of good quality embryo in each cycle, while the fertile control refers to patients who had a history of at least one live birth with no related comorbidities (Bastu et al., 2019 (link)). The platform and series matrix files were all downloaded.