Plasmids pIRES2-EGFP and PX330 were kindly provided by Prof. Zhanjun Li (Jilin University, China). Plasmid pEGFP-MNDAL-N1 was constructed previously in our lab [2 (link)].
FITC-labeled goat anti-rabbit IgG (H + L), HRP-labeled goat anti-mouse IgG (H + L), HRP-labeled goat anti-rabbit IgG (H + L), DAPI, Enhanced BCA Protein Assay Kit, BeyoECL Plus kit, Cell lysis buffer for Western and IP, and Caspase3/8/9 ELISA Kit were purchased from Beyotime (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased from Bioster (Wuhan, China).
TRIM21 rabbit polyclonal antibody, mouse P53 monoclonal antibody, and mouse Bcl-2 polyclonal antibodies were purchased from Proteintech (Wuhan, China). Lipofectamine 3000 Transfection Reagent and PTI-MEM were from Thermo Scientific (USA). Porcine TNF-α and IL-6 ELISA Kits were purchased from Absin (Shanghai, China). Rabbit PCV2 Cap antibody was from Biorbyt (Wuhan, China), and the rabbit anti-PCV2 Cap was prepared previously in the lab (1:200) [26 (link)]. Mouse IFN-α antibody, rabbit IFN-β antibody, mouse IFN-γ antibody, and mouse MNDA antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TIANamp Virus DNA/RNA Kit, TRNzol-A+ Reagent, 2× Taq plus PCR Mix, and FastKing-RT SuperMix kit were from Tiangen (Beijing, China). Luna® Universal qPCR Master Mix (SYBR Green) was from New England Biolabs (NEB, Ipswich, MA, USA).
FITC-labeled goat anti-rabbit IgG (H + L), HRP-labeled goat anti-mouse IgG (H + L), HRP-labeled goat anti-rabbit IgG (H + L), DAPI, Enhanced BCA Protein Assay Kit, BeyoECL Plus kit, Cell lysis buffer for Western and IP, and Caspase3/8/9 ELISA Kit were purchased from Beyotime (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased from Bioster (Wuhan, China).
TRIM21 rabbit polyclonal antibody, mouse P53 monoclonal antibody, and mouse Bcl-2 polyclonal antibodies were purchased from Proteintech (Wuhan, China). Lipofectamine 3000 Transfection Reagent and PTI-MEM were from Thermo Scientific (USA). Porcine TNF-α and IL-6 ELISA Kits were purchased from Absin (Shanghai, China). Rabbit PCV2 Cap antibody was from Biorbyt (Wuhan, China), and the rabbit anti-PCV2 Cap was prepared previously in the lab (1:200) [26 (link)]. Mouse IFN-α antibody, rabbit IFN-β antibody, mouse IFN-γ antibody, and mouse MNDA antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TIANamp Virus DNA/RNA Kit, TRNzol-A+ Reagent, 2× Taq plus PCR Mix, and FastKing-RT SuperMix kit were from Tiangen (Beijing, China). Luna® Universal qPCR Master Mix (SYBR Green) was from New England Biolabs (NEB, Ipswich, MA, USA).
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