Pyrola corbieri Levl was collected in Guiyang city, Guizhou Province, PR China, in June 2018 and was authenticated by Prof. Qingwen Sun, Guizhou University of Traditional Chinese Medicine. A voucher specimen (No. 201806LTC) was deposited at the Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences. DEAE-cellulose was supplied by Shanghai Hengxin Chemical Reagent Co., Ltd. (Shanghai, China), and Sephacryl-300 was purchased from Amersham Biosciences (Uppsala, Sweden). Galacturonic acid and the standards dextrans T-40 (Mw: 40 kDa), T-25 (Mw: 25 kDa), T-12 (Mw: 12 kDa), T-5 (Mw: 5 kDa) and T-1 (Mw: 1 kDa) were provided by Sigma Chemical Co. (MO, USA). D-mannose, D-glucose, D-galactose, D-xylose, D-fucose, L-rhamnose and L-arabinose were purchased from Shanghai Chemical Reagents Company (Shanghai, China). The pheochromocytoma cell line (PC12) was purchased from Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). The acetylcholinesterase kit and Pierce BCA protein kit were purchased from Shanghai Genmed Scientifics Co., Ltd. (Shanghai, China). GAP-43 (7B10) antibody was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). An Enhanced ECL Chemiluminescent Substrate Kit was purchased from PerkinElmer Life Sciences (Boston, MA, USA). Green fluorescent protein (GFP) transgenic mice (18 days gestation) were a kind gift from Professor Zhenggang Yang (Institute of Brain Science, Fudan University, Shanghai, China). Trypsin was acquired from Gibco-Life Technologies (Grand Island, NY, USA). DNAase and Trolox were purchased from Sangon Biotech Co., Ltd. (Shanghai, China).
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