P. aeruginosa isolates were grown in LB broth (Neogen, USA) for 24 h at 30°C. Then, swarming agar plates containing 1% glucose (Synth, Brazil), 0.5% peptone (Acumedia, USA), 0.2% yeast extract (Bacto, Difco, USA), and 0.5% agar (Acumedia, USA) were equilibrated to room temperature and inoculated at the center with 10 µL of a P. aeruginosa suspension containing 108 colony-forming units (CFU)/mL in the presence and absence (control) of subinhibitory concentrations (½ MIC) of bio-AgNPs. Plates were incubated without inversion for 24 h at 30°C (Norizan et al., 2013 (link)).
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