Diagnostic Criteria and Therapeutic Approaches for Myeloid Malignancies

This study was approved by Institutional Ethics Committee of the Affiliated People's Hospital of Jiangsu University, and written informed consents were obtained from all participants. A total of 60 healthy donors were used as controls. The diagnosis and classification of 99 MDS, 212 AML and 91 patients with CML were established according to the revised French–American–British (FAB) classification and the 2008 World Health Organization (WHO) criteria 16, 17. The IPSS scores were utilized to classify the risk groups of MDS 18. Our study focused on BM mononuclear cells (BMMNCs) extracted as reported previously 19. The treatment for MDS patients with lower IPSS scores (Low/Int‐1) was symptomatic and supportive treatment with/without thalidomide, whereas patients with higher IPSS scores (Int‐2/High) received chemotherapy included aclacinomycin, cytarabine, granulocyte colony stimulating factor together with symptomatic and supportive treatment. Patients with AML received chemotherapy including induction therapy and subsequent consolidation treatment 20, 21. For non‐M3 patients, induction therapy was one or two courses of daunorubicin combined with cytarabine. Subsequent consolidation treatment included high‐dose cytarabine, mitoxantrone with cytarabine and homoharringtonine combined with cytarabine. Meanwhile, for M3 patients, induction therapy was oral all‐trans retinoic acid (ATRA) together with daunorubicin in combination with cytarabine. Maintenance therapy was oral mercaptopurine, oral methotrexate and oral ATRA over 2 years.