Comprehensive Blood and Serum Analysis

Blood samples were collected into EDTA Vacutainers (BD Biosciences, San Jose, CA, USA) for a complete blood cell count (CBC) using an automated analyzer (Bayer ADVIA 120; Bayer Diagnostics, Tarrytown, NY, USA). Serum was isolated from whole blood collected without an anticoagulant, centrifuged (1000g for 10 min) and submitted for a full biochemical profile (Cobas c501; Roche Diagnostics International, Risch, Switzerland, http://www.roche-diagnostics.ch). Lymphocytes were phenotyped (CD4, CD8, CD21) exactly as previously described [22 (link), 23 (link)].

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Arzi B., Peralta S., Fiani N., Vapniarsky N., Taechangam N., Delatorre U., Clark K.C., Walker N.J., Loscar M.R., Lommer M.J., Fulton A., Battig J, & Borjesson D.L. (2020). A multicenter experience using adipose-derived mesenchymal stem cell therapy for cats with chronic, non-responsive gingivostomatitis. Stem Cell Research & Therapy, 11, 115.

Publication 2020

EDTA Vacutainers Bayer ADVIA 120 Cobas c501

Anticoagulant Blood Diagnostics Edta Lymphocytes Serum

Corresponding Organization : University of California, Davis

Other organizations : Cornell University, Veterinary Medical Teaching Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Complete blood cell count (CBC)
Biochemical profile
Lymphocyte phenotypes (CD4, CD8, CD21)

control variables

EDTA Vacutainers used for blood collection
Automated analyzer (Bayer ADVIA 120) used for CBC
Serum isolated from whole blood collected without anticoagulant
Centrifugation at 1000g for 10 minutes
Biochemical profile analyzed using Cobas c501 (Roche Diagnostics)
Lymphocyte phenotyping methods previously described [22, 23]

controls

No positive or negative controls explicitly mentioned

Annotations

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