C57BL/6J mice (9-weeks-old) were kept in a pathogen-free facility and maintained under a 12 h light–dark cycle at 22° C. 3 mice were fed ad libitum with a high-fat diet (HFD; TD88137 Harlan Teklad) and 3 mice were fed ad libitum with a regular diet (RD). VAT were excised and isolation of preadipocytes [63 (link)]. Animal care and experimental procedures were approved by the Ethics Committee in Animal Experimentation of West China Hospital, Sichuan University, Chengdu, China (record #: 2019014A).
In the GEO database GSE28598 data set, Smad3+/+ mice (WT) and Smad3−/− mice (KO) were fed with a regular diet (RD) or 55% high fat diet (HFD) for 8 weeks. Diet-induced obese (DIO) mice were intraperitoneally injected with 1.5 mg/kg body weight of control 13C4 antibody (IgG) or anti-TGF-β antibody (1D11) three times a week for 8 weeks [22 (link)].
In the GEO database GSE28598 data set, Smad3+/+ mice (WT) and Smad3−/− mice (KO) were fed with a regular diet (RD) or 55% high fat diet (HFD) for 8 weeks. Diet-induced obese (DIO) mice were intraperitoneally injected with 1.5 mg/kg body weight of control 13C4 antibody (IgG) or anti-TGF-β antibody (1D11) three times a week for 8 weeks [22 (link)].
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