Quantitative Analysis of Saccharides
Corresponding Organization :
Other organizations : Fractionnation of AgroResources and Environment, Biodiversité et Biotechnologie Fongiques, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, IFP Énergies nouvelles, Architecture et Fonction des Macromolécules Biologiques, Biopolymères Interactions Assemblages, Joint Genome Institute, Los Alamos National Laboratory, Centre de Recherches sur les Macromolécules Végétales
Variable analysis
- Mono-, oligo-saccharides and their corresponding aldonic acids present in S2
- Non-oxidized oligosaccharides (Megazyme, Bray, Ireland) used as standards
- Gluconic acid (Megazyme) and corresponding C1-oxidized standards (from DP2 to DP6) produced from non-oxidized cello-oligosaccharides using a cellobiose dehydrogenase treatment
- Concentrations of mono-, oligo-saccharides and their corresponding aldonic acids present in S2
- Ionic chromatography (HPAEC) analysis method as already described [37]
- Non-oxidized oligosaccharides (Megazyme, Bray, Ireland) used as positive controls
- Gluconic acid (Megazyme) and corresponding C1-oxidized standards (from DP2 to DP6) used as positive controls
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