Mono-, oligo-saccharides and their corresponding aldonic acids present in S2 were analysed by ionic chromatography (HPAEC) as already described [37 (link)], using non-oxidized oligosaccharides (Megazyme, Bray, Ireland) as standards. Gluconic acid (Megazyme) and corresponding C1-oxidized standards (from DP2 to DP6) were produced from non-oxidized cello-oligosaccharides using a cellobiose dehydrogenase treatment [37 (link)].
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