IHC were performed as described in previous study (26 (link)). Briefly, mouse lung tissue sections were incubated with an antibody collagen-1 (GB111364, diluted 1: 500; Servicebio, Whhan, China) or a-SMA (GB11022-3, 1:1000 dilution; Servicebio) overnight at 4°C, followed incubating by HPR-conjugated secondary antibody (GB23303 or GB23301, 1:1000 dilution; Servicebio). Diaminobenzidine was used as the substrate. Integrated optical density of collagen-1 and a-SMA were detected by using Image-Pro Plus software.
Xu X., Wang Y., Luo X., Gao X., Gu W., Ma Y., Xu L., Yu M., Liu X., Liu J., Wang X., Zheng T., Mao C, & Dong L. (2023). A non-invasive strategy for suppressing asthmatic airway inflammation and remodeling: Inhalation of nebulized hypoxic hUCMSC-derived extracellular vesicles. Frontiers in Immunology, 14, 1150971.
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