Maternal Body Composition and Adipokines

Body composition was measured by air-displacement plethysmography using the Bod Pod Gold Standard system (Bod Pod 2007 A, Life Measurement, Concord, CA, software versions 4.2.0 and 5.2.0.) using gestational-age specific equations according to our previously described protocol^{29 (link)}. Leptin and adiponectin were analysed at the Clinical Chemistry Laboratory, Sahlgrenska University Hospital (accredited in accordance with the International Standard ISO 15189:2007). Leptin (Human Leptin Quantikine, R&D Systems, Minneapolis, MN; interassay coefficient of variation, 8.0% at 9 μg/l), the soluble leptin receptor (sOB-R) (Human Leptin R Quantikine, R&D Systems) and adiponectin (Human Adiponectin ELISA kit, Millipore, Billerica, MA; interassay coefficient of variation, 7.0% at 10.5 mg/l) were analysed using ELISA. sOB-R measurements were conducted for a subset of women, i.e. the women that were included in the full PONCH study of both mother and child²¹ ; this resulted in 84 NW women and 33 OB women that had complete sOB-R measurements in both T1 and T3.