Pharmacological Manipulation of Synaptic Plasticity

Myristoylated ZIP, pep2m, and scrambled peptide were acquired from Tocris Bioscience; prostaglandin E₂ (PGE₂) was obtained from Cayman Chemical Company; and nerve growth factor (NGF) was from R&D Systems. ZIP, pep2m and scrambled peptide stock solutions were prepared in sterile 0.9% saline and diluted from this stock solution for injection. PGE₂ stock solutions were prepared in 100% ethanol and diluted in sterile saline for injection. Doses used in this study were based on our previous work (Asiedu et al., 2011 ) and other publications (Shi et al., 2001 (link), Pastalkova et al., 2006 (link), Shema et al., 2007 (link)). ZIP is not a selective inhibitor of aPKCs (Lee et al., 2013 (link), Volk et al., 2013 (link)). pep2m interferes with GluA2 binding to NSF and the effect of the peptide is absent in GluA2 KO mice, suggesting it is a specific tool peptide (Shi et al., 2001 (link)).

Free full text: Click here

Baptista-de-Souza D., Tavares-Ferreira D., Megat S., Sankaranarayanan I., Shiers S., Flores C.M., Ghosh S., Luiz Nunes-de-Souza R., Canto-de-Souza A, & Price T.J. (2020). Sex differences in the role of atypical PKC within the basolateral nucleus of the amygdala in a mouse hyperalgesic priming model. Neurobiology of Pain, 8, 100049.

Publication 2020

pep2m prostaglandin E2 (PGE2)

0 9 saline Cayman Ethanol Mice Nerve growth factor Peptide Prostaglandin e2 Saline Sterile

Corresponding Organization : The University of Texas at Dallas

Other organizations : Universidade Federal de São Carlos, Janssen (United States), Yale University, Universidade Estadual Paulista (Unesp)

Top 5 similar protocols

Variable analysis

independent variables

Myristoylated ZIP
Pep2m
Scrambled peptide
Prostaglandin E2 (PGE2)
Nerve growth factor (NGF)

dependent variables

Not explicitly mentioned

control variables

Sterile 0.9% saline
100% ethanol

controls

Positive control: Not specified
Negative control: Scrambled peptide

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!