Cell Culture Conditions for Diverse Cell Lines

MDCK (canine), 293T (human), A549 (human), 1321N1 (human), EidNi/41 (bat; Eidolon helvum) (54 (link)), and CarperAEC.B-3 (bat; Carollia perspicillata) (55 (link)) cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco Life Technologies). HAP-1 cells (Horizon Discovery, Austria) were cultured in Iscove's modified Dulbecco's medium (IMDM) supplemented with the same additives, as well as 200 μM GlutaMAX (Gibco Life Technologies). The HAP-1 cell line engineered by CRISPR/Cas9 to have a 2-bp deletion in the PIK3R2 gene (encoding p85β) was purchased from Horizon Discovery (catalog no. HZGHC003292c006). All cells were maintained at 37°C with 5% CO₂.

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Turkington H.L., Juozapaitis M., Tsolakos N., Corrales-Aguilar E., Schwemmle M, & Hale B.G. (2018). Unexpected Functional Divergence of Bat Influenza Virus NS1 Proteins. Journal of Virology, 92(5), e02097-17.

Publication 2017

penicillin streptomycin HAP-1 cells GlutaMAX HAP-1 cell line

Canine Cell line Cells Crispr Cultured medium Fetal bovine serum Gene deletion Human Penicillin Streptomycin

Corresponding Organization :

Other organizations : University of Zurich, University of Freiburg, University Medical Center Freiburg, Universidad de Costa Rica

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Variable analysis

independent variables

Cell lines used: MDCK (canine), 293T (human), A549 (human), 1321N1 (human), EidNi/41 (bat; Eidolon helvum), CarperAEC.B-3 (bat; Carollia perspicillata), and HAP-1 (human)

dependent variables

Not explicitly mentioned

control variables

Cell culture media: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco Life Technologies)
HAP-1 cells cultured in Iscove's modified Dulbecco's medium (IMDM) supplemented with the same additives, as well as 200 μM GlutaMAX (Gibco Life Technologies)
All cells maintained at 37°C with 5% CO2

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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