Example 10

LacZ and Luciferase Modified RNA Cardiac Transfection and Translation in a Citrate Saline Buffer

As depicted, 75 μg of LacZ encoding modified RNA with cardiac injections was transfected and translated in approximately 10% of the left ventricle (FIGS. 11A, 11B, 11C, and 11D). RNA in situ hybridization for luciferase modified RNA revealed staining expression in the myocardium at the site of injection (FIGS. 11E and 11F) and correlative luciferase protein expression shown via immunohistochemical analysis in the serial section (FIG. 11G).

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