Comprehensive Hepatic Cancer Analysis

The Annexin V-FITC/PI kit was purchased from Invitrogen Corporation. Survivin, Ki67, livin, bcl-2, p53, VEGF, and MVD immunohistochemistry kits were purchased from Cell Signaling Technology Corporation. HepG2 cell line was provided by the Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences. PHRE-Egr1-HSV-TK was constructed previously [1 (link)]. Hepatic cancer tissues of the control group, the radionuclide group, the MFH group, the radionuclide-gene group, and the radionuclide-gene-MFH group came from the previous study [1 (link)].

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Lin M., Zhou C., Huang J., Tian W., Yu H., Jiang X., Ye J., Guo T., Shi Y., Xiao Y., Bian X, & Feng X. (2018). The Possible Mechanisms of HSV-TK/Hyperthermia Combined with 131I-antiAFPMcAb-GCV Nanospheres to Treat Hepatoma. Analytical Cellular Pathology (Amsterdam), 2018, 8941908.

Publication 2018

Annexin V-FITC/PI kit Survivin livin bcl-2 VEGF

Annexin v fitc Bcl 2 Chinese Egr1 Gene Hepatic cancer Hepg2 cell line Immunohistochemistry Livin Radionuclide Survivin Tissues Vegf

Corresponding Organization : Nantong University

Other organizations : Wuhan Institute of Technology

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Variable analysis

independent variables

Radionuclide treatment
MFH treatment
Radionuclide-gene treatment
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dependent variables

Apoptosis (Annexin V-FITC/PI)
Expression of Survivin, Ki67, livin, bcl-2, p53, VEGF, and MVD

control variables

Control group

positive controls

PHRE-Egr1-HSV-TK (previously constructed)

negative controls

Not explicitly mentioned

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