MERS-CoV Spike Protein Epitope Mapping
Variable analysis
- Restimulation with 2 µg/mL of a pool of 275 peptides corresponding to MERS-CoV spike protein
- Frequencies of IFN-γ+ cells
- IFN-γ secreting splenocytes reported as the average of spot forming cells (SFCs) per million splenocytes
- Number of splenocytes per well (500,000, 250,000, and 125,000 cells)
- Incubation time (18–20 h) and temperature (37 °C)
- Use of Mouse IFN-γ ELISpot kit following manufacturer's instructions
- Counting of total number of spots in each well using an AID ELISpot reader
- Not explicitly mentioned
- Further duplicate wells were plated with 250,000 cells from each sample and left without peptide restimulation
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