Inhibition of Cytokine-Induced Epithelial Growth

Cytokines previously reported to be able to induce EG (30 (link), 31 (link)) were inhibited by injection of antibody or biological inhibitors. Anakinra [Kineret from Sobi DIN 02245913; anti-IL-1 at 25 μg/g mouse as used in (46 (link))], etanercept [Enbrel from Amgen, DIN 02242903; anti–TNF-α at 15 μg/g mouse as used in (47 (link))], tocilizumab [Actemra from Roche, DIN 02350092; anti-IL-6R at 5 μg/g mouse as used in (48 (link))], anti–IL-3 (aIL3 from Abcam, ab9726; at 2.5 μg/g mouse as recommended by the product sheet], anti–G-CSF (from R&D Systems, MAB414; 10 μg per injection as used in (49 (link))], and anti–GM-CSF (from Abcam, ab9741; at 10 μg/g mouse as recommended by the product sheet] were each administered in 40-μl intraperitoneal doses to separate mice 12 hours before vaccination, at vaccination, and at 24 and 48 hours after vaccination until euthanasia at 72 hours after vaccination for flow cytometry of splenocytes.

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Brook B., Harbeson D.J., Shannon C.P., Cai B., He D., Ben-Othman R., Francis F., Huang J., Varankovich N., Liu A., Bao W., Bjerregaard-Andersen M., Schaltz-Buchholzer F., Sanca L., Golding C.N., Larsen K.L., Levy O., Kampmann B., Tan R., Charles A., Wynn J.L., Shann F., Aaby P., Benn C.S., Tebbutt S.J., Kollmann T.R, & Amenyogbe N. (2020). BCG vaccination–induced emergency granulopoiesis provides rapid protection from neonatal sepsis. Science translational medicine, 12(542), eaax4517.

Publication 2020

Enbrel Actemra ab9726 MAB414 ab9741

Actemra Anakinra Antibody injection Biological Cytokines Enbrel Etanercept Euthanasia Flow cytometry G csf Gm csf Il 6r Inhibitors Kineret Mice Tnf α Tocilizumab Vaccination

Corresponding Organization : The Kids Research Institute Australia

Other organizations : St. Paul's Hospital, Prevention of Organ Failure, Bandim Health Project, Statens Serum Institut, Odense University Hospital, University of Southern Denmark, Boston Children's Hospital, Harvard University, Broad Institute, MRC Unit the Gambia, London School of Hygiene & Tropical Medicine, Weill Cornell Medical College in Qatar, University of Florida, University of Melbourne

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Variable analysis

independent variables

Injection of antibody or biological inhibitors: Anakinra (anti-IL-1), etanercept (anti-TNF-α), tocilizumab (anti-IL-6R), anti-IL-3, anti-G-CSF, and anti-GM-CSF

dependent variables

Flow cytometry of splenocytes

control variables

Dose of antibodies and inhibitors: Anakinra at 25 μg/g mouse, etanercept at 15 μg/g mouse, tocilizumab at 5 μg/g mouse, anti-IL-3 at 2.5 μg/g mouse, anti-G-CSF at 10 μg per injection, and anti-GM-CSF at 10 μg/g mouse
Timing of administration: 12 hours before vaccination, at vaccination, and at 24 and 48 hours after vaccination until euthanasia at 72 hours after vaccination

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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