The cell viability was assessed by (CCK8, Promega) as previously described (30 (link)). Stably expressing PCDH20 cells or vector were cultured in 200 μl of a 96-well plate (2,000 cells per well) with 10% FBS-contained 1,640 for 24, 48, and 72h. The cells were co-cultured in 100 μl of serum-free 1640 containing 10 μl CCK8 at 37°C for 2h. Microplate reader (Multiskan MK3, Thermo Fisher Scientific, Schwerte, Germany) was used to measure absorbance at 450 nm.
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