Determining Thermal Stability of H-24 and H-20

Samples containing 0.5 mM of H-24 or H-20 in the presence or absence of ssDNA1 were prepared in 37 mM citric acid and 126 mM Na₂HPO₄ (pH 6) buffer, or 100 mM Tris (pH 9) buffer. The T_t was obtained by measuring the absorbance of the samples at 380 nm as a function of temperature and pH in a temperature-controlled UV–vis spectrophotometer (Cary 300 UV − vis, Agilent) as previously described^{46 (link)}. The T_t was obtained by taking the maximum in the first derivative of the absorbance as a function of temperature of triplicate samples.

Free full text: Click here

Díez Pérez T., Tafoya A.N., Peabody D.S., Lakin M.R., Hurwitz I., Carroll N.J, & López G.P. (2024). Isolation of nucleic acids using liquid–liquid phase separation of pH-sensitive elastin-like polypeptides. Scientific Reports, 14, 10157.

Publication 2024

Cary 300 UV − vis

Corresponding Organization :

Other organizations : University of New Mexico, Center for Global Health

Top 5 similar protocols

Variable analysis

independent variables

Concentration of H-24 or H-20 (0.5 mM)
Presence or absence of ssDNA1

dependent variables

Transition temperature (Tt) measured by absorbance at 380 nm as a function of temperature and pH

control variables

PH (6 or 9)
Buffer composition (37 mM citric acid and 126 mM Na2HPO4, or 100 mM Tris)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!