Detailed methodology can be found in the Supplementary Information . In short, luciferase reporter gene assay, immunoprecipitation and immunoblotting analysis, purification of recombinant proteins, in vitro kinase assays, and cell migration assay were performed, as previously described14 (link),21 (link),22 (link).
Protocol detail
Characterization of Signaling Pathways
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Assays
Cell migration assay
Immunoblotting analysis
Immunoprecipitation
Kinase
Luciferase
Recombinant proteins
Reporter gene
Corresponding Organization : The University of Texas MD Anderson Cancer Center
Other organizations : Jiaxing University, Model Animal Research Center, Nanjing University
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Variable analysis
independent variables
- None explicitly mentioned
- Luciferase reporter gene activity
- Protein-protein interactions measured by immunoprecipitation and immunoblotting
- Kinase activity measured by in vitro kinase assays
- Cell migration measured by cell migration assay
- None explicitly mentioned
- Positive controls: Not specified
- Negative controls: Not specified
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