Human pancreatic cancer cell line, AsPC-1, was cultured in RPMI with 10% fetal bovine serum (FBS), 1% L- Glutamine, and 1% penicillin streptomycin(P/S). Panc-1 was cultured in DMEM with 10% FBS and 1% P/S. Both AsPC-1 and Panc-1 were purchased from ATCC.
Pancreatic tumors from patients with pancreatic ductal adenocarcinoma were collected under IRB12-1108 and IRB13-1149, confirmed to be tumor based on pathologic assessment, and developed into organoid culture according to established protocols (Romero-Calvo et al., 2019 (link)). Four different organoids, U0118-8, U049MAI, U114SOK, and U123M15-T, were investigated. For the optimal culture, derived organoids were embedded in growth factor reduced Matrigel and cultured in Intesticult complete media, supplemented with A83-01, fibroblast growth factor 10, gastrin I, N-acetyl-L-cysteine, nicotinamide, and B27 supplement, primocin. Tocris Y-27632 dihydrochloride, a selective p160 ROCK inhibitor, was added when thawing the organoids (Romero-Calvo et al., 2019 (link)).
Pancreatic tumors from patients with pancreatic ductal adenocarcinoma were collected under IRB12-1108 and IRB13-1149, confirmed to be tumor based on pathologic assessment, and developed into organoid culture according to established protocols (Romero-Calvo et al., 2019 (link)). Four different organoids, U0118-8, U049MAI, U114SOK, and U123M15-T, were investigated. For the optimal culture, derived organoids were embedded in growth factor reduced Matrigel and cultured in Intesticult complete media, supplemented with A83-01, fibroblast growth factor 10, gastrin I, N-acetyl-L-cysteine, nicotinamide, and B27 supplement, primocin. Tocris Y-27632 dihydrochloride, a selective p160 ROCK inhibitor, was added when thawing the organoids (Romero-Calvo et al., 2019 (link)).
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