Metabolomics and Isotope Tracing Analysis

Metabolomics and isotope tracing analyses were conducted using an Agilent 1290 Infinity Liquid Chromatography (LC) System (Agilent) coupled to a Q-TOF 6545 mass spectrometer (MS, Agilent). Targeted analysis, isotopologues extraction, and natural isotope abundance correction were performed with Agilent Profinder B.10.00 (Agilent) as previously described (11 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Sagt C.M., Kleizen B., Verwaal R., de Jong M.D., Müller W.H., Smits A., Visser C., Boonstra J., Verkleij A.J, & Verrips C.T. (2000). Introduction of an N-Glycosylation Site Increases Secretion of Heterologous Proteins in Yeasts. Applied and Environmental Microbiology, 66(11), 4940-4944.

Publication 2023

Agilent 1290 Infinity Liquid Chromatography (LC) System Agilent Profinder B.10.00

Corresponding Organization : Stanford University

Top 5 similar protocols

Variable analysis

independent variables

Metabolomics and isotope tracing analyses

dependent variables

Measured outcomes

control variables

Kept constant to ensure valid results

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!