Antibody Validation for Drosophila Studies

The following antibodies were used in this study: Mouse anti-Drosophila Rab7 hybridoma supernatant (1:5) and goat anti-Drosophila golgin245 (both developed and validated by S. Munroe [Riedel et al., 2016 (link)] and obtained from the Developmental Studies Hybridoma Bank). Rabbit anti-Drosophila Rab5 (1: 250, Ab31261; Abcam). Rat anti-tdTomato (1:500, EST203; Kerafast). Mouse anti-GFP (1:500, 1181446000; Sigma-Aldrich). Mouse anti-insect cathepsin L antibody (1:500, MAB22591; R&D Systems). Mouse anti-tubulin antibody (1:2,000, T9026; Sigma-Aldrich). The guinea pig anti-Drosophila scattered (Vps54) antibody was generated by and obtained from R. Sinka (Fári et al., 2016 (link); 1:400). Secondary antibodies for immunohistochemistry were anti-mouse, goat, rabbit, or rat labeled by Alexa 488, 555, or 647 (1:1,000; Thermo Fisher Scientific). Secondary antibodies for Western blot were anti-mouse HRP (1:1,000, 115-035-146; Jackson), anti-guinea pig HRP (1:500, A5545; Sigma-Aldrich), or anti-mouse IR-Dye 680 LT (1:20,000; LI-COR).