Oxidative
folding of
KIIIA peptides was performed as described previously.29 (link) Oxidative folding of S-m3-huwentoxin-IV was
accomplished by glutathione-assisted folding at 25 °C overnight
under the following conditions: 15 μM reduced peptide in 0.1
M Tris-HCl (pH 8.0, Amresco, Solon, OH), 10% (v/v) isopropanol (Chem-Supply
Pty. Ltd.), and 5 mM reduced and 1 mM oxidized glutathione (Sigma-Aldrich).
The reaction was quenched by decreasing the pH to 2 using an ACN/TFA/H2O mixture [1/1/1 (v/v/v)].
folding of
KIIIA peptides was performed as described previously.29 (link) Oxidative folding of S-m3-huwentoxin-IV was
accomplished by glutathione-assisted folding at 25 °C overnight
under the following conditions: 15 μM reduced peptide in 0.1
M Tris-HCl (pH 8.0, Amresco, Solon, OH), 10% (v/v) isopropanol (Chem-Supply
Pty. Ltd.), and 5 mM reduced and 1 mM oxidized glutathione (Sigma-Aldrich).
The reaction was quenched by decreasing the pH to 2 using an ACN/TFA/H2O mixture [1/1/1 (v/v/v)].
