Bordetella bronchiseptica RB50 wild-type (Bb) and RB50 mutant strain, herein BbDbtrS [55 (link)], were grown on plates of Difco Bordet-Gengou agar (BD, cat. 248200) supplemented with 10% sheep defibrinated blood and 20 μg/mL concentration of streptomycin, as previously described [55 (link),65 (link)].
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