Phocaeicola dorei 5_1_36 and Lachnoclostridium symbiosum WAL-14673 were obtained from BEI Resources (HM-29 and HM-319). For inoculations and initial tests, both microorganisms were cultured in Reinforced Clostridium Media (RCM; Becton, Dickinson, Franklin Lakes, NJ) supplemented with 0.5 g/liter of l -cysteine (RCM-cys; Loba Chemie, India). All incubations were performed at 37°C for 24 to 48 h in an anaerobic jar (Anaerocult; Merck, Darmstadt, Germany) with anaerobic packs (Gaspak EM; Becton, Dickinson, Franklin Lakes, NJ, USA). mZMB medium was prepared as in reference 48 (link).
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