Gibson Assembly Reagents Preparation

Enzymes for Gibson Assembly preparation were purchased in NEB (Ipswich, MA, USA). Media were from Helicon (Moscow, Russia). Oligonucleotides were made by Syntol (Moscow, Russia). All chemicals were of analytical purity. Hydrogen peroxide (H₂O₂) was obtained from Ferraine (Moscow, Russia). Mitomycin C (MitC) and methyl methanesulfonate (MMS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 2,2′-bis(bicyclo[2.2.1] heptane) (BBH) was synthetized as in previous work [32 (link)]. All test solutions and their dilutions were prepared immediately before use.

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Kessenikh A.G., Novoyatlova U.S., Bazhenov S.V., Stepanova E.A., Khrulnova S.A., Gnuchikh E.Y., Kotova V.Y., Kudryavtseva A.A., Bermeshev M.V, & Manukhov I.V. (2021). Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters. International Journal of Molecular Sciences, 22(17), 9571.

Publication 2021

Mitomycin C (MitC) methyl methanesulfonate (MMS)

Dilutions Enzymes H2o2 Heptane Methyl methanesulfonate Mitomycin c Oligonucleotides

Corresponding Organization : National Research University Higher School of Economics

Other organizations : National Research Center for Hematology Russian Academy of Medical Sciences, Kurchatov Institute, Genetika, Federal Scientific Center for Biological Systems and Agricultural Technologies of the Russian Academy of Sciences, Russian Academy of Sciences, A.V. Topchiev Institute of Petrochemical Synthesis

Top 5 similar protocols

Variable analysis

independent variables

Hydrogen peroxide (H2O2)
Mitomycin C (MitC)
Methyl methanesulfonate (MMS)
2,2'-bis(bicyclo[2.2.1] heptane) (BBH)

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Enzymes for Gibson Assembly preparation
Media
Oligonucleotides
Chemicals of analytical purity

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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