Plasma Aβ Quantification for Neurological Research

Blood for preparation of EDTA plasma was drawn through venipuncture at the Amsterdam UMC, VU University Medical Center. Plasma tubes were centrifuged at 1800×g for 10 min within 1 h of collection, and plasma was aliquoted in 0.5-mL portions in polypropylene tubes and stored at − 80 °C. Samples were first thawed at the Amsterdam UMC for the quantitative determination of plasma concentrations of Aβ_1–40 and Aβ_1–42 with the SIMOA Human Neurology 3-Plex A assay kit (Quanterix, Lexington, MA) using the SIMOA HD-1 analyzer [16 (link)]. Thereafter, 200 μl of plasma per subject was shipped from Amsterdam to Bochum on dry ice.

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Stockmann J., Verberk I.M., Timmesfeld N., Denz R., Budde B., Lange-Leifhelm J., Scheltens P., van der Flier W.M., Nabers A., Teunissen C.E, & Gerwert K. (2020). Amyloid-β misfolding as a plasma biomarker indicates risk for future clinical Alzheimer’s disease in individuals with subjective cognitive decline. Alzheimer's Research & Therapy, 12, 169.

Publication 2020

SIMOA Human Neurology 3-Plex A assay kit SIMOA HD-1 analyzer

Assay Dry ice Edta Human Plasma Polypropylene Venipuncture

Corresponding Organization :

Other organizations : Ruhr University Bochum, Vrije Universiteit Amsterdam, Amsterdam Neuroscience, Amsterdam University Medical Centers

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma concentrations of Aβ₁–₄₀ and Aβ₁–₄₂

control variables

Blood was drawn through venipuncture
Plasma tubes were centrifuged at 1800×g for 10 min within 1 h of collection
Plasma was aliquoted in 0.5-mL portions in polypropylene tubes
Samples were stored at − 80 °C
Samples were first thawed at the Amsterdam UMC

controls

No positive or negative controls were explicitly mentioned in the protocol.

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