Synthesis and Evaluation of Berberine Derivatives

Berberine (B1), berberrubine (B2), 9-O-isoprenylberberrubine bromide (B3), 9-O-gernylberberrubine bromide (B4), and 9-O-farnesylberberrubine bromide (B5) were synthesized and provided by Dr. Jin-Yi Wu [7 (link)]. Dimethyl sulfoxide (DMSO), propidium iodide, crystal violet, chloroquine, rapamycin, 3-methyladenine (3-MA), paclitaxel (Taxol), and transforming growth factor beta (TGF-β) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies such as phospho-CDK2 (2351-1; Epitomics; Burlingame, CA, USA), CDK4 (sc-601; Santa Cruz Biotechnology; Dallas, TX, USA), p27 (GTX100446; GeneTex; Irvine, CA, USA), caspase-3 (#9662; Cell Signaling Technology; Beverly, MA, USA), PARP (#9542; Cell Signaling Technology), GAPDH (GTX100118; GeneTex), LC3 (AP1802a; Abgent; San Diego, CA, USA), p62 (#5114; Cell Signaling Technology), N-cadherin (#4061; Cell Signaling Technology), E-cadherin (610182; BD Biosciences; Bedford, MA, USA), claudin-1 (#4933; Cell Signaling Technology), and snail (#3879; Cell Signaling Technology) as well as secondary antibodies such as rabbit antimouse antibody (GTX26728; GeneTex) and goat antirabbit antibodies (GTX213110; GeneTex) were purchased and used in this study. All chemicals and biochemicals used in this study were of analytical grade.

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Chang J.M., Wu J.Y., Chen S.H., Chao W.Y., Chuang H.H., Kam K.H., Zhao P.W., Li Y.Z., Yen Y.P, & Lee Y.R. (2021). 9-O-Terpenyl-Substituted Berberrubine Derivatives Suppress Tumor Migration and Increase Anti-Human Non-Small-Cell Lung Cancer Activity. International Journal of Molecular Sciences, 22(18), 9864.

Publication 2021

Dimethyl sulfoxide (DMSO), propidium iodide crystal violet chloroquine rapamycin 3-methyladenine (3-MA), paclitaxel (Taxol), transforming growth factor beta (TGF-β) caspase-3 GAPDH N-cadherin E-cadherin claudin-1 snail

3 methyladenine Antibodies Berberine Berberrubine Bromide Caspase 3 Cdk2 Chloroquine Claudin 1 Crystal violet Dimethyl sulfoxide E cadherin Gapdh Goat N cadherin Paclitaxel Propidium iodide Rabbit Rapamycin Snail Taxol Tgf β

Corresponding Organization : Kaohsiung Medical University

Other organizations : Chia-Yi Christian Hospital, Asia University, National Chiayi University, Min-Hwei College of Health Care Management, Kaohsiung Medical University Chung-Ho Memorial Hospital

Top 5 similar protocols

Variable analysis

independent variables

Berberine (B1)
Berberrubine (B2)
9-O-isoprenylberberrubine bromide (B3)
9-O-gernylberberrubine bromide (B4)
9-O-farnesylberberrubine bromide (B5)

dependent variables

Cell viability/proliferation
Apoptosis
Autophagy
Epithelial-mesenchymal transition (EMT)

control variables

Dimethyl sulfoxide (DMSO)
Propidium iodide
Crystal violet
Chloroquine
Rapamycin
3-methyladenine (3-MA)
Paclitaxel (Taxol)
Transforming growth factor beta (TGF-β)

positive controls

Paclitaxel (Taxol)
Transforming growth factor beta (TGF-β)

negative controls

Dimethyl sulfoxide (DMSO)

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