Antibodies and their manufacturers were: rat anti-BPLF1 [56 (link)] (WB 1:1600, 2E5 supernatant) was from Helmholtz Zentrum (2E5); mouse anti-FLAG clone M2 (WB 1:4000, IF 1:400; F1804) and rabbit anti-LC3B (WB 1:1000, IF 1:200; L7543) were from Sigma-Aldrich; goat anti-FLAG (1:400; ab1257) and rabbit anti-SQSTM1/p62 (IP 1–2 μg/mg of lysate; ab101266), rabbit anti-GFP (WB 1:2000, ab290) and rabbit IgG isotype control (ab172730) were from Abcam; mouse anti-SQSTM1/p62 (WB 1:1000 IF 1:200; 610,832) was from BD Biosciences; rabbit anti-SQSTM1/p62 (WB 1:1000, 8025S) was from Cell Signaling Technology; mouse anti-HA clone 12 CA5 (WB 1:2000; 11,583,816,001) from Roche; mouse IgG isotype control (14–4732-82) was from Invitrogen; Alexa Fluor 488-, 555-, 594- and 647-conjugated secondary antibodies were from Thermo Fisher (A21206, A31570, A11032 and A21447, respectively).
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