Quantifying Bacterial Gene Expression

Analysis of bacterial gene expression by qPCR has been described previously [21 (link)]. S. Typhimurium were grown in LB containing 0.3M NaCl [26 (link)] or low phosphate and magnesium-containing medium (LPM, pH 5.8) [28 (link)] for induction of T3SS-1 or T3SS-2, respectively. RNA extraction and qPCR were performed as described above. The data were analyzed using the comparative Ct method (Applied Biosystems). Transcription of the target gene was normalized to the levels of gyrA mRNA.

Free full text: Click here

Takemura M., Haneda T., Idei H., Miki T, & Okada N. (2021). A Salmonella type III effector, PipA, works in a different manner than the PipA family effectors GogA and GtgA. PLoS ONE, 16(3), e0248975.

Publication 2021

comparative Ct method

Bacterial Gene expression analysis Gene transcription Magnesium Mrna Nacl Phosphate

Corresponding Organization : Kitasato University

Top 5 similar protocols

Variable analysis

independent variables

Growth conditions: LB containing 0.3M NaCl or low phosphate and magnesium-containing medium (LPM, pH 5.8)

dependent variables

Transcription of the target gene

control variables

GyrA mRNA levels (used for normalization)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!