Western blotting was performed as described previously [13 (link)]. The following antibodies were used: CCN1 antibody (Abcam), β-actin antibody (Santa Cruz Biotechnology), Bcl-xL antibody (Cell Signaling Technology), c-Myc antibody (Cell Signaling Technology), Bax antibody (Santa Cruz Biotechnology), MEK antibody (Cell Signaling Technology), phospho-MEK antibody (Ser217/221, Cell Signaling Technology), ERK antibody (Cell Signaling Technology), phospho-ERK antibody (Thr202/Tyr204, Cell Signaling Technology), β-catenin antibody (Cell Signaling Technology), phospho-β-catenin antibody (Ser33/Ser37/Thr41, Cell Signaling Technology), and Survivin antibody (Cell Signaling Technology).
Gel electrophoresis and transfer as well as the chemiluminescence detection were conducted using the Bio-Rad Laboratories system.
Nuclear protein was extracted using a nuclear protein extraction kit (Beyotime, China), and an anti-Histone H1 antibody (Santa Cruz Biotechnology) was used as the loading control.
Gel electrophoresis and transfer as well as the chemiluminescence detection were conducted using the Bio-Rad Laboratories system.
Nuclear protein was extracted using a nuclear protein extraction kit (Beyotime, China), and an anti-Histone H1 antibody (Santa Cruz Biotechnology) was used as the loading control.
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