Cell lines possessing the ecotropic retroviral receptor and the tetracycline repressor were generated and used in RNA interference library screening, shRNA toxicity assays and cDNA complementation studies as described4 (link). DLBCL cell lines were assigned to the ABC or GCB subtypes by gene expression profiling (ref.4 (link); Supplemental Fig. 14 ). shRNA screening results are given in Supplemental Tables 1 and 3 , and shRNA sequences are listed in Supplemental Tables 2 and 3 . Specific shRNA-mediated mRNA and protein knockdown was documented (Fig. 2C ; Supplemental Fig. 6A , 15 ). IKK reporter lines were engineered to express an IκBα-Photinus luciferase fusion and Renilla luciferase27 (link). TIRF imaging of the BCR was based on previously described techniques12 .
Tumor biopsies were obtained prior to treatment from patients with de novo DLBCL28 (link), gastric MALT lymphoma, and Burkitt’s lymphoma. All samples were studied according to a protocol approved by the National Cancer Institute Institutional Review Board.
Tumor biopsies were obtained prior to treatment from patients with de novo DLBCL28 (link), gastric MALT lymphoma, and Burkitt’s lymphoma. All samples were studied according to a protocol approved by the National Cancer Institute Institutional Review Board.
