The MOB-suite v. 1 database contains 12 091 complete plasmids, and due to the increased number of plasmid sequences made available since the original publication, we expanded the database using new data from the NCBI utilizing the same approach described in the supplementary materials of the MOB-suite paper [14 (link)]. The NCBI Entrez nucleotide database was queried in November 2019 with the query ‘plasmid’ AND ‘complete sequence’ AND ‘bacteria [organism]’. The results were then filtered for sequences between 1500 to 400 000 bp in length, with ‘plasmid’ as the genetic compartment and limited to the INSDC (International Nucleotide Sequence Database Collaboration). This yielded an initial set of 33 875 sequences that were then typed using MOB-typer v. 2.1.0 (Table S1, available with the online version of this article). Records were excluded due to the presence of any of the following terms in the title or description: gene, cds, protein, transposon, insertion, protein, region, operon, pseudogene, integrase, transposase, integron, partial, shotgun. The remaining set of 23 280 sequences was then merged with the MOB-suite v.1 database plasmids, which were then de-duplicated by clustering plasmids that had a Mash v. 2.2.2 [17 (link)] distance of 0 and selecting a single representative for subsequent analyses (Table S2). A priority was given to those plasmids that were part of the initial construction of the MOB-suite clusters, which resulted in a total of 17 779 records. The database exhibits a strong bias towards plasmids from Enterobacteriaceae (35 %) as shown in a Krona plot of the plasmid dataset taxonomic composition (Fig. S1). This has the consequence that the threshold optimization may not be fully representative of the underrepresented taxonomic groups.