Synovial tissue sections were dewaxed with 4% xylene and dehydrated with gradient alcohol.31 (link) For H&E staining, tissues were stained with H&E staining kit (toluidine blue, Keygen Biotech. Nanjing, China). For Masson, tissues were stained with Masson trichrome staining kit (toluidine blue, Keygen Biotech. Nanjing, China). For Safranin O staining, tissues were stained with Safranin O solution (ScyTek, Logan, UT, USA). All staining was performed according to the manufacturer’s instruction, and the morphological changes were observed with an optical microscope (BX51; Olympus Corp., Tokyo, Japan).
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Histological Examination of Synovial Tissue
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Other organizations : Anyang Hospital of Traditional Chinese Medicine
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Variable analysis
independent variables
- Staining methods (H&E, Masson, Safranin O)
- Morphological changes
- Dewaxing with 4% xylene
- Dehydration with gradient alcohol
- Staining performed according to manufacturer's instructions
- Optical microscope (BX51; Olympus Corp., Tokyo, Japan) used for observation
- No positive or negative controls were explicitly mentioned in the input.
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