Immunohistochemistry was performed as previously described.33 (link) Primary antibodies used in this article are described in Supplementary Table S2 . Alexa Fluor 488 goat anti-mouse, 488 goat anti-rabbit, 546 goat anti-rabbit, and 546 goat anti-mouse secondary antibodies (Molecular Probes, Invitrogen) were all used at 1:200 dilution. Nuclei were visualized by counterstaining with DAPI (1:10,000 dilution). Samples were mounted in 60% glycerol in PBS. Images were taken at 20× and 40× on an inverted fluorescent microscope (Eclipse Ti-U; Nikon Instruments). At least six sections were analyzed on each slide and for each antibody.
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